How to Operate Vacuum Filter
The concept and principles of Vacuum filtration are designed to promote very much expedited filtration process compared to syringe filtering, the process after setup is hands free which anyone who has pushed 100mls through a syringe filter will appreciate.
The basic gist of the concept is that we create a pressure differential between the inside of the sterile collector vessel and the outside, thereby PULLING the filtrate (you precious gear) through the filter
Here’s a basic picture to illustrate the concept.
The picture above shows the basic setup with lab gear – if you were able to find a small enough filter mesh AND properly sterilize the equipment this kind of setup would work fine.
We prefer to work with the disposable gamma irradiated sterile filters,look like this: